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Antibodies bound to the blocking peptide no more bind for the epitope around the target protein. By comparing the staining with the blocked antibody versus the antibody on your own, you can see which staining is distinct.
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This guidebook outlines further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
This guideline outlines even more possible optimization of cellular and immunoassay parameters to be sure the best possible results are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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Blocking peptides are peptides that bind specially on the target antibody and block antibody binding. These peptide typically consists of the epitope recognized via the antibody.
Concentrate on Information and facts Cyclin E belongs to the really conserved cyclin relatives, whose customers are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which contribute into the temporal coordination of each and every mitotic party. Cyclin E sorts a posh with and functions being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S phase.
Overexpression of the gene is observed in several tumors, which results in chromosome instability, and so could add to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two further splice variants were being noted but specific nucleotide sequence info is just not yet obtainable.
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